Aptamer Details

Back to Search Results

Epithelial Cell Adhesion Molecule (EpCAM) SYL3C (ID# 7980)

Aptamer Chemistry: DNA

Target: Cancer Cells

Antigen/Target Category: Cells

Affinity (Kd): 38 nM (reported value)

Binding Conditions/Buffer: 1 x PBS (pH-7.4) and 5 mM MgCl2

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: 38 nM against MDA-MB-231 breast cancer cell line and 38 nM against Kato III human gastric carcinoma cell line

Sequence ():

Length: 48

Molecular Weight: 14838.6

Extinction Coefficient: 443700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Selection of DNA Aptamers against Epithelial Cell Adhesion Molecule for Cancer Cell Imaging and Circulating Tumor Cell Capture Yanling Song, Zhi Zhu, Yuan An, Weiting Zhang, Huimin Zhang, Dan Liu, Chundong Yu, Wei Duan, and Chaoyong James Yang Analytical Chemistry 2013 85 (8), 4141-4149 DOI: 10.1021/ac400366b

Have your aptamer oligo synthesized ORDER