Aptamer Details

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FANA R8-3 (ID# 8173)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: Chimeric

Target: SARS-CoV-2 S Protein

Antigen/Target Category: Protein

Affinity (Kd): 68.1 nM (reported value)

Binding Conditions/Buffer: Buffer: 200 µl PBS (137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4) pH: 7.4 Incubation time: 30 mins

Binding Temp: 23°C

Refolding Protocol: About 200 pmol of 5′ 32P-labeled FANA starting pool was heat to 90⁰C then snap-cooled on ice. The material was then incubated with 20 pmol of SARS-COV-2 RBD protein that had been attached to Dynabeads™ using the C-terminal His-tag. Incubations were in 200 µl PBS (137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 mM KH2PO4, pH 7.4) for 30 min with agitation at room temperature.

Comments: These are not the common 2'-F-RNA nucleotides, these are 2'-Fluoro-Arabino Nucleic Acid (FANA) nucleotides.

Sequence ():

Length: 79

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Ferreira-Bravo, I. A. and J. J. DeStefano. 2021. Xeno-nucleic Acid (XNA) 2'-Fluoro-Arabino Nucleic Acid (FANA) Aptamers to the Receptor Binding Domain of SARS-CoV-2 S Protein Block ACE2 Binding. bioRxiv https://doi.org/10.1101/2021.07.13.452259

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