Aptamer Details

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GL21.T (ID# 9015)

Aptamer Chemistry: RNA

Target: Axl

Antigen/Target Category: Protein

Affinity (Kd): 12 nM (reported value)

Binding Conditions/Buffer: filter binding analysis with the soluble extracellular domain of human Axl, Dtk, and Mer as targets (R&D Systems, Minneapolis, MN), was performed by incubating 1 nmol/l of radiolabeled aptamers with 1, 3.2, 10, 32, 100, 320, and 1,000 nmol/l of EC-Axl, EC-Dtk, or EC-Mer

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 34

Molecular Weight: 10297.6 g/mole

Extinction Coefficient: 319300 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Cerchia L, Esposito CL, Camorani S, Rienzo A, Stasio L, Insabato L, Affuso A, de Franciscis V. Targeting Axl with an high-affinity inhibitory aptamer. Mol Ther. 2012 Dec;20(12):2291-303. doi: 10.1038/mt.2012.163. Epub 2012 Aug 21. PMID: 22910292; PMCID: PMC3519989.

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