Aptamer Details

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Glycated Hemoglobin Aptamer (ID# 7969)

Aptamer Chemistry: DNA

Target: Glycated Hemoglobin

Antigen/Target Category: Protein

Affinity (Kd): 7.3 nM (reported value)

Binding Conditions/Buffer: Aptamer binding took place in human blood sample produced by centrifuging 100 µL of whole human blood, collecting the pellet of red blood cells, resuspending the pellet in 50 µL of deionized water, and straining the resulting suspension.

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 72

Molecular Weight: 22588.62

Extinction Coefficient: 694400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

J Li et al. On chip, aptamer-based sandwich assay for detection of glycated hemoglobins via magnetic beads. Biosensors and Bioelectronics 79 (2016): 887-893

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