Aptamer Details

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GVI-1 (ID# 8081)

Aptamer Chemistry: DNA

Target: GCRV-infected CIK

Antigen/Target Category: Cells

Affinity (Kd): 220.86 nM (reported value)

Binding Conditions/Buffer: Binding Buffer [10% FBS] from Life Technologies.

Binding Temp: 4°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Grass Carp Reovirus Infected Cytokine-Induced Killer Cells (GCRV-infected CIK cells). Grass carp (Ctenopharyngon idellus) kidney cells (CIK).

Sequence ():

Length: 90

Molecular Weight: 27920.1

Extinction Coefficient: 874000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Yu, Qing et al. “Generation and characterization of aptamers against grass carp reovirus infection for the development of rapid detection assay.” Journal of fish diseases vol. 44,1 (2021): 33-44. doi:10.1111/jfd.13265

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