Aptamer Details

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Heparanase (1.5M NaCl) (ID# 7790)

Aptamer Chemistry: DNA

Target: Heparanase (HPSE1)

Antigen/Target Category: Protein

Affinity (Kd): 12 nM nM (reported value)

Binding Conditions/Buffer: Salt Solution: 100 mM NaCl, 20 mM KCl, 5 mM MgCl₂

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Selected in the presence of 1.5 M NaCl.
This aptamer showed significant inhibition of heparanase in a Matrigel cell invasion assay, outperforming the polyclonal anti-herparanase antibody used as a positive control.
A 30-nt truncated version of this aptamer including only the stem-loop structure showed similar binding affinity but diminished inhibitory activity.

Sequence ():

Length: 72

Molecular Weight: 22348.62

Extinction Coefficient: 727000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Simmons, SC, et al. Development of novel single-stranded nucleic acid aptamers against the pro-angiogenic and metastatic enzyme heparanase (HPSE1). PLoS One (2012) 7:e37938.

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