Aptagen, LLC
Hepatitis C Virus Dependent RNA Polymerase (B.2) (ID# 7571)
RNA
Hepatitis C Virus Dependent RNA Polymerase
Protein
1.5 nM (reported value)
Binding buffer: 20 mM Tris-HCl (pH 7.0), 1 mM dithiothreitol (DTT), 0.25 U/ul RNasin, 100 ng/ul BSA, 250 mM NaCl, 0.03% n-octyl-B-D-glucopyranoside, 5 mM MgCl2, and 2.0% glycerol
23°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprGprAprUprGprCprUprUprCprGprGprCprAprUprCprCprCprCprGprAprAprGprCprCprGprCprUprAprUprGprGprAprCprCprAprGprUprGprGprCprGprCprGprGprCprUprUprCprGprGprCprCprCprGprAprCprGprGprAprGprUprGprGprUprAprCprCprGprCprUprUprCprGprGprCprGprGprUprAprCprGprUprAprAprGprCprUprUprGprGprGp-3'
92
29744.87
868400
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Biroccio et al. "Selection of RNA Aptamers That Are Specific and High-Affinity Ligands of the Hepatitis C Virus RNA-Dependent RNA Polymerase." J. Virol>, 76 (2002):3688-3696.
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