Aptamer Details

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HIV-1 Tat protein (ID# 7730)

Aptamer Chemistry: RNA

Target: HIV-1 Tat protein

Antigen/Target Category: Protein

Affinity (Kd): 120 pM (reported value)

Binding Conditions/Buffer: Binding Buffer: 50 mM Tris-HCl (pH 7.8) and 50 mM KCl

Binding Temp: r.t.°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 37

Molecular Weight: 11784.08

Extinction Coefficient: 352600

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

R Yamamoto et al. A novel RNA motif that binds efficiently and specifically to the Tat protein of HIV and inhibits the trans-activation by Tat of transcription in vitro and in vivo. Genes to Cells 5(2000):371-388

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