Aptamer Details

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HIV Reverse Transcriptase (PF1) (ID# 7781)

Aptamer Chemistry: DNA

Target: HIV RT

Antigen/Target Category: Protein

Affinity (Kd): 80 nM (reported value)

Binding Conditions/Buffer: 50 mM Tris-HCl, pH 8, 1mM DTT, 80 mM KCl, 6 mM MgCl₂ for ten minutes.

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 30

Molecular Weight: 9326.14

Extinction Coefficient: 300100

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Y-T Lai and J DeStefano. DNA aptamers to human immunodeficiency virus reverse transcriptase selected by a primer-free SELEX method: characterization and comparison with other aptamers. Nucleic Acid Therapeutics 22(2012): 162-176.

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