Aptamer Details

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Human Immunoglobulin G (IgG) (Apt 8) (ID# 7461)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA

Target: hlgG-Fc

Antigen/Target Category: Protein

Affinity (Kd): 75 nM (reported value)

Binding Conditions/Buffer: Binding Buffer (145mM NaCl, 5.4 mM KCl, 0.8mM MgCl, 1.8 mM CaCl, and 20 mM Tris-HCl (pH 7.6)) Modifications at the 5' and 3' end have been made by the authors for various testing.

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Specific for Human IgG, subclasses hIgG1 through hIgG4.
Does not bind to mouse, rat, rabbit, bovine, chicken, canine, feline, and guinea pig IgG.

Comments: This aptamer binds to the Fc domain of hIgG.
See also the modified version of this aptamer, 8-2.

Sequence ():

Length: 23

Molecular Weight: 7442.54

Extinction Coefficient: 226600

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Miyakawa, Shin, et al. "Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G." RNA Journal 14,(2008): 1154-1163.

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