Aptamer Details

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Human Influenza A virus H3N2 (P30-10-16) (ID# 7527)

Aptamer Chemistry: RNA

Target: Human Influenza A virus H3N2

Antigen/Target Category: Cells

Affinity (Kd): 0.188 nM (reported value)

Binding Conditions/Buffer: Binding Buffer (20 nM HEPES/KOH (pH 7?ú4), 105 mM NaCl) PIPES buffer (5 mM PIPES/NaOH, 145 mM NaCl (pH 7?ú5)) Acidic buffer (145 mM NaCl, 20 mM sodium citrate, 1 U RNase inhibitor µl-1 (pH 5?ú0))

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 74

Molecular Weight: 23621.16

Extinction Coefficient: 710100

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Gopinath, S., et al. "An RNA aptamer that distinguishes between closely related human influenza viruses and inhibits haemagglutinin-mediated membrane fusion." Journal of General Virology, 87(2005): 479-487.

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