Aptamer Details

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HupB-4T (ID# 7989)

Aptamer Chemistry: DNA

Target: HupB

Antigen/Target Category: Protein

Affinity (Kd): 1.72 µM (reported value)

Binding Conditions/Buffer: 10 mM Tris pH 7.5, 10 mM MgCl2, 50 mM KCl, 25 mM NaCl

Binding Temp: 23°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: The aptamer forms a G-Quadruplex

Sequence ():

Length: 21

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Miyakawa, Shin, et al. "Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G." RNA Journal 14, (2008): 1154-1163. Kalra, P., et al. "G-Quadruplex-Forming DNA Aptamers Inhibit the DNA-Binding Function of HupB and Mycobacterium tuberculosis Entry into Host Cells." Molecular Therapy: Nucleic Acids. Vol. 13. December 2018. https://doi.org/10.1016/j.omtn.2018.08.011

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