Aptagen, LLC
Insulin-Linked Polymorphic Region Two-Repeat (ILPR2) (ID# 7863)
DNA
Insulin
Peptide
N/A nM (reported value)
Tris buffer (20 mM Tris, 5mM KCL, pH 7.3)
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
In capillary protein capture, this aptamer captured 5 times more insulin than thrombin.
Each repeat forms a G-quartet.
5'-dApdCpdApdGpdGpdGpdGpdTpdGpdTpdGpdGpdGpdGpdApdCpdApdGpdGpdGpdGpdTpdGpdTpdGpdGpdGpdGp-3'
28
8911.82
283900
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Connor A C, McGown L B, et al. (2006) Insulin Capture by an Insulin-Linked Polymorphic Region G-Quadruplex DNA Oligonucleotide. J Am Chem Soc. 128: 4986-4991.
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