Aptamer Details

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Internalin A of Listeria monocytogenes (ID# 7769)

Aptamer Chemistry: DNA

Target: Internalin A (InlA)

Antigen/Target Category: Protein

LOD: 10³ CFU/mL (reported value)

Binding Conditions/Buffer: TBS: Tris 10 mM, NaCl 0.85%, pH 8.0

Binding Temp: 4°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Limited cross-reactivity with non-virulent strains of Listeria (L innocua, L grayi, L ivanovii, L seeligeri, and L welshimeri), E coli, S enterica, L acidophilus, S aureus, and P aeruginos.

Sequence ():

Length: 47

Molecular Weight: 14811.63

Extinction Coefficient: 470400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

S.H. Ohk et al. Antibody-aptamer functionalized fibre-optic biosensor for specific detection of Listeria monocytogenes from food. J. Appl. Microbiol. 109(2010): 808-817.

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