Aptamer Details

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J7 (ID# 9000)

Aptamer Chemistry: DNA

Target: CD3ε

Antigen/Target Category: Protein

Affinity (Kd): 3.06 ± 0.485 nM (reported value)

Binding Conditions/Buffer: incubating 10.8 nmol denatured HPLC (high-performance liquid chromatography)-purified single-stranded DNA (ssDNA) library with 1.0 × 107 cells in a final volume of 500 μL.

Refolding Protocol: The DNA library suspended in 250 μL RPMI was denatured by heating at 95°C for 5 min, followed by folding for 45 min at 25°C in order to allow the formation of proper secondary structures.

Sequence ():

Length: 85

Molecular Weight: 26516.1 g/mole

Extinction Coefficient: 837200 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Zumrut HE, Batool S, Argyropoulos KV, Williams N, Azad R, Mallikaratchy PR. Integrating Ligand-Receptor Interactions and In Vitro Evolution for Streamlined Discovery of Artificial Nucleic Acid Ligands. Mol Ther Nucleic Acids. 2019 Sep 6;17:150-163. doi: 10.1016/j.omtn.2019.05.015. Epub 2019 Jun 4. PMID: 31255977; PMCID: PMC6606840.

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