Aptagen, LLC
Kanamycin B (K8) (ID# 7523)
RNA
Kanamycin B
Small Organic
180 nM (reported value)
Binding Buffer: 140 mM NaCl, 5 mM KCl, lmM MgCl2, and 20 mM HEPES at pH 7.5
20°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Strong binding to tobramycin was observed (Kd = 11.6 nM).
Binding to kanamycin A, neomycin, and paromomycin was much weaker (Kds > 1000 nM).
Binding to kanamycin A, neomycin, and paromomycin was much weaker (Kds > 1000 nM).
5'-rGprGprGprAprGprCprUprCprGprGprUprAprCprCprGprAprAprUprUprCprUprCprGprCprCprCprUprAprUprAprGprGprGprGprUprGprUprUprGprAprGprGprGprAprAprAprUprGprUprGprUprGprCprGprAprCprAprAprGprGprUprGprCprGprGprUprGprGprCprCprAprGprAprAprCprUprUprUprUprCprGprUprUprCprUprCprAprUprCprAprAprAprAprGprCprUprUprUprGprCprAprGprAprGprGprAprUprCprCprUprUp-3'
111
35819.5
1095000
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kwon et al., "In Vitro Selection of RNA against Kanamycin B." Molecules and Cells, 11 (2001): 303-311.
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