Aptamer Details

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LipL32 DNA Aptamer LepDapt-5a (ID# 8409)

Aptamer Chemistry: DNA

Target: LipL32 Protein

Antigen/Target Category: Protein

Affinity (Kd): 33.97 nM (reported value)

Binding Conditions/Buffer: 10 mM HEPES-KOH [pH 7.4], 150 mM NaCl

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Yeast tRNA was introduced in SELEX section as the competitor to DNA candidates.

Comments: LepDapt-5a was proven to be the most potent aptamer against LipL32 protein. It is a prolific diagnostic agent which can be used in developing potential diagnostic platforms with designing specific hybrid-heterodimeric aptamer to detect pathogenic Leptospira.

Sequence ():

Length: 28 bp

Molecular Weight: 18.48 kDa

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Yeoh TS, Tang TH, Citartan M. Isolation of a novel DNA aptamer against LipL32 as a potential diagnostic agent for the detection of pathogenic Leptospira. Biotechnol J. 2023 Mar;18(3):e2200418. doi: 10.1002/biot.202200418. Epub 2022 Dec 8. PMID: 36426669.

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