Aptamer Details

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Microcystin-LR (AN6) (ID# 7828)

Aptamer Chemistry: DNA

Target: Microcystin-LR

Antigen/Target Category: Small Organic

Affinity (Kd): 50 nM (reported value)

Binding Conditions/Buffer: Binding Buffer: 50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM MgCl₂

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Binds to congener microcystin-LA with three-fold reduced affinity (Kd of 158). No binding observed to microcystin-YR.

Sequence ():

Length: 60

Molecular Weight: 18167.79

Extinction Coefficient: 557700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Ng A, Zourob M, et al. (2012) Selection, characterization and biosensing application of high affinity congener-specific microcystin-targeting aptamers. Environ. Sci. Technol. 46: 10697-10703.

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