Aptamer Details

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Mouse Transferrin Receptor (FB4) (ID# 7816)

Aptamer Chemistry: RNA

Target: Mouse Transferrin Receptor (mTfR)

Antigen/Target Category: Protein

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: RNA binding buffer (20 mM NaPO₄, pH 7.4/120 mM NaCl/2.5 mM KCl/0.12 mM MgSO₄/40 mM Hepes, pH 7.4)

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Does not bind to human TfR cells.

Comments: The RNA aptamer-streptavidin conjugate was mouse-specific. Human cells needed to first be transfected with mouse transferrin receptor in order to be taken up by human cells. The FB4 aptamer does not inhibit transferrin suggesting that binding is not competitive.

Sequence ():

Length: 79

Molecular Weight: 25651.39

Extinction Coefficient: 765200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

E F Neufeld et al. Aptamer-based endocytosis of a lysosomal enzyme. Proc. Natl. Acad. Sci. USA 105(2008): 15908-15913

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