Aptamer Details

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MRP1Apt (3) (ID# 8998)

Aptamer Chemistry: RNA

Target: MRP1

Antigen/Target Category: Protein

Affinity (Kd): 50 nM (reported value)

Binding Conditions/Buffer: The pre-cleared RNA pool was incubated with the bead-coupled MRP1 peptide and washed three times with washing buffer that contained 150 mmol/l NaCl, 2 mmol/l CaCl2, 20 mmol/l HEPES (pH 7.4), so as to remove the non-interacting RNA species. The MRP-1 peptide-binding RNA species were extracted after 30 minutes of incubation through phenol:chloroform:isoamyl alcohol (25:24:1), followed by reverse transcription and PCR amplification

Binding Temp: 37°C

Refolding Protocol: 37 C for 30 minutes. Buffer: 150 mmol/l NaCl, 2 mmol/l CaCl2, 20 mmol/l HEPES (pH 7.4), 0,01% BSA binding buffer

Specificity: Affinity for CEM-NKR negative cells is >3000 nM.

Sequence ():

Length: 63

Molecular Weight: 19015 g/mole

Extinction Coefficient: 563500 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Soldevilla MM, Villanueva H, Casares N, Lasarte JJ, Bendandi M, Inoges S, López-Díaz de Cerio A, Pastor F. MRP1-CD28 bi-specific oligonucleotide aptamers: target costimulation to drug-resistant melanoma cancer stem cells. Oncotarget. 2016 Apr 26;7(17):23182-96. doi: 10.18632/oncotarget.8095. PMID: 26992239; PMCID: PMC5029619.

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