Aptamer Details

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MT32 protein of Apple Stem Pitting Virus (ASPV) (ID# 7560)

Aptamer Chemistry: DNA

Target: MT32 coat protein

Antigen/Target Category: Protein

Affinity (Kd): 55 nM (reported value)

Binding Conditions/Buffer: Selection Buffer: PBS-T (10 mM Na₂HPO₄, 2 mM KH₂PO₄, 2.7 mM KCl, 137 mM NaCl, and 0.05%Triton-X; pH 7.5) with added 0.1 μg/ml poly-dIdC and 1 μg/ml BSA.

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Binds to the PSA-H coat protein with similar affinity (83 nM).

Comments: Most likely forms a g-quartet.

Sequence ():

Length: 40

Molecular Weight: 12619.23

Extinction Coefficient: 380300

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Balogh, et al. "Selection and versatile application of virus-specific aptamers." FASEB Journal, 24(2010): 4187-4195.

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