Aptamer Details

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nDNA-547 (ID# 8141)

Aptamer Chemistry: DNA

Target: nano-sized DNA

Antigen/Target Category: Small Organic

Affinity (Kd): 363 ± 135 nM nM (reported value)

Binding Conditions/Buffer: 7 M urea, 10 mM EDTA, 300 mM NaOAc, pH 5.2

Binding Temp: 26°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 72

Molecular Weight: 21887.2

Extinction Coefficient: 666800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Ogawa, A., & Itoh, Y. (2020). In Vitro Selection of RNA Aptamers Binding to Nanosized DNA for Constructing Artificial Riboswitches. ACS Synthetic Biology. doi:10.1021/acssynbio.0c00384

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