Aptagen, LLC
Neuropeptide Y (DP3) (ID# 7806)
DNA
Neuropeptide Y (NPY)
Peptide
370 nM (reported value)
Binding Buffer: 0.9 mM KH2P4, Na2HPO4, 70 mM NaCL, 1.3 mM KCl, 1% heparin, 0.4 g/l of streptavidin, pH 7.6.
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
The aptamer did not bind to the similar human pancreatic polypeptide (hPP; ~50% sequence homology).
Truncation of the target peptide showed that this aptamer targets the C-terminus of neuropeptide Y.
Competition assays showed that this aptamer inhibits binding of neuropeptide Y to the Y2 receptor (IC50 of 73 nM). Less inhibition was observed for the Y1 and Y5 receptors (IC50 of 328 nM and 416 nM, respectively).
Competition assays showed that this aptamer inhibits binding of neuropeptide Y to the Y2 receptor (IC50 of 73 nM). Less inhibition was observed for the Y1 and Y5 receptors (IC50 of 328 nM and 416 nM, respectively).
5'-rGprGprGprAprGprAprAprAprGprGprGprAprAprGpnCpnUpnUprGprAprGp nCprAprGpnCprAprGprGprAprGprGprGpnCpnCprGprGpnCprGpnUpnUprAprGprGprGpnUpnUprAprGpnCprGprAprGpnCpnCprGprApnUpnUprGprAprAp rAprGprAprAprGprAprAprGprGprAprApnCprGprAprGpnCprGpnUprApnCprGprGprApnUpnCpnCprGprApnUpnCp -3'
90
45033.34
928300
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
D. Proske et al. A Y2 receptor mimetic aptamer directed against neuropeptide Y. J. Biol. Chem. 277(2002):11416-11422.
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