Aptamer Details

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NoPv1 (ID# 8199)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: Peptide

Target: PvCesA2

Antigen/Target Category: Protein

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: V. Vinifera cv. Pinot noir variety

Binding Temp: 22-25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: N/A

Comments: Peptide Sequence: RLTAQCRL Peptide was separately tested in context of sterilized distilled water, LB medium, and pea agar media.

Sequence ():

Length: 8

Molecular Weight: 959.53

Extinction Coefficient: 211167

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Colombo, M., Masiero, S., Rosa, S., Caporali, E., Toffolatti, S. L., Mizzotti, C., Tadini, L., Rossi, F., Pellegrino, S., Musetti, R., Velasco, R., Perazzolli, M., Vezzulli, S., & Pesaresi, P. (2020). Nopv1: A synthetic antimicrobial peptide aptamer targeting the causal agents of grapevine downy mildew and potato late blight. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-73027-x

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