Aptamer Details

Back to Search Results

OS-7.9 Aptamer (ID# 8190)

Aptamer Chemistry: DNA

Target: Human Osteosarcoma MG-63

Antigen/Target Category: Cells

Affinity (Kd): 12.8 nM (reported value)

Binding Conditions/Buffer: Phosphate-Buffered Saline (PBS) 1X with 4.5 mg/mL glucose [Acros], 5 mM MgCl2 [VWR]. 1 mg/mL bovine serum albumin (BSA). The buffers were filtered through a 0.22 um syringe filter.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: The OS-7.9 aptamer displayed significant binding towards human colon colorectal adenocarcinoma (Widr) and human lung carcinoma (A549), implying the presence of at least one common marker.

Comments: The aptamer shows high binding affinity at 4C and 37C.

Sequence ():

Length: 85

Molecular Weight: 26177

Extinction Coefficient: 836200

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Tsogtbaatar, K., Sousa, D. A., Ferreira, D., Tevlek, A., Aydın, H. M., Çelik, E., & Rodrigues, L. (2021). In vitro selection of DNA aptamers against human osteosarcoma. Investigational New Drugs. doi:10.1007/s10637-021-01161-y

Have your aptamer oligo synthesized ORDER