Aptamer Details

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Paramylon-binding Fluorogenic Peptide (ID# 7994)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: Peptide

Target: Paramylon in E. gracilis

Antigen/Target Category: Protein

Affinity (Kd): Oct-60 µM (reported value)

Binding Conditions/Buffer: AF-6 medium , mannitol (0.3 M)

Binding Temp: 37°C

Refolding Protocol: N/A

Specificity: <10 fold against Kuzuko starch

Comments: Peptide Aptamer Sequence: Met-Thr-Thr-Cys-Ala-Arg-Arg-Phe-Arg-Ukn-Ile-Gly-Trp-Arg-Phe-Cys-Ser-Trp Identified by ribosome display, synthesized by solid-phase chemistry

Sequence ():

Length: 18

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

1. Takanori Maeno, Takanori Uzawa, Izumi Kono, Kazunori Okano, Takanori Iino, Keisuke Fukita, Yuki Oshikawa, Taro Ogawa, Osamu Iwata, Takuro Ito, Kengo Suzuki, Keisuke Goda, Yoichiroh Hosokawa. Targeted delivery of fluorogenic peptide aptamers into live microalgae by femtosecond laser photoporation at single-cell resolution. Scientific Reports, 2018; 8 (1) DOI: 10.1038/s41598-018-26565-4

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