Aptamer Details

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Phorate (SS2-55) (ID# 7677)

Aptamer Chemistry: DNA

Target: Phorate

Antigen/Target Category: Small Organic

Affinity (Kd): 1.11 µM (reported value)

Binding Conditions/Buffer: Selection buffer used in binding affinity assays: 300mM NaCl, 50 mM KCl, 10 mM MgCl₂, 50 mM Tris/HCl, and pH 8.3

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: This aptamer binds preferentially to the organophosphates phorate, profenofos, benzoate and omethoate. It has reduced binding to imidacloprid, acetamiprid, chlorpyrifos, methamidophos, dichlorvos, parathion, dimethoate, monocrotophos and phoxim.

Comments: "A molecular beacon (5' FAM-CTGCACAAGAATCGCTGCAG-3') was designed, which was partly complementary with the middle constant nucleotides-sequence of the selected ssDNAs."

Sequence ():

Length: 55

Molecular Weight: 16990.09

Extinction Coefficient: 526900

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Wang, L, et al. (2012). Selection of DNA aptamers that bind four organophosphorus pesticides. Biotechnol Lett, 2012(34), 869-874. doi: 10.1007/s10529-012-0850-6

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