Aptamer Details

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Protective Antigen (PA) of Bacilus anthracis (MMS_105) (ID# 7646)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: Peptide

Target: Protective Antigen (PA) of Bacilus anthracis

Antigen/Target Category: Cells

Affinity (Kd): 19.1 nM (reported value)

Binding Conditions/Buffer: Binding Buffer: PBSB (PBS buffer plus 0.5% BSA), 4°C

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: 71.6% Binding to 150 nM PA. The clone with the highest binding percentage was MMS_128 at 89.8% (K_d=54.9 nM).

Sequence ():

Length: 15

Molecular Weight: 1967.27

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Kogot et al. "Screening of Peptide Libraries against Protective Antigen of Bacillus anthracis in a Disposable Microfluidic Cartridge." PloS One, 6(11): e26925. doi:10.1371/journal.pone.0026925

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