Aptamer Details

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R10C5 (ID# 8114)

Aptamer Chemistry: DNA

Target: Legionella pneumophila (Lp)

Antigen/Target Category: Other

Affinity (Kd): 116 nM (reported value)

Binding Conditions/Buffer: 1X PBS with 0.1 mg/ml salmon sperm DNA, 1% bovine serum albumin, and 0.05% Tween 20

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 69

Molecular Weight: 21213.8

Extinction Coefficient: 658800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Saad M, Chinerman D, Tabrizian M, Faucher SP. Identification of two aptamers binding to Legionella pneumophila with high affinity and specificity. Sci Rep. 2020 Jun 4;10(1):9145. doi: 10.1038/s41598-020-65973-3. PMID: 32499557; PMCID: PMC7272621.

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