Aptamer Details

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R5A1 (ID# 9101)

Aptamer Chemistry: RNA

Target: interleukin-10 receptor

Antigen/Target Category: Protein

Affinity (Kd): 12 nM (reported value)

Binding Conditions/Buffer: Purified RNA was folded by heating to 82 °C 5′ in selection buffer (150 mmol/l NaCl, 20 mmol/l KCl, 20 mmol/l HEPES) and cooling slowly to room temperature

Binding Temp: 37 °C°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 34

Molecular Weight: 10823.6 g/mole

Extinction Coefficient: 329400 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Berezhnoy A, Stewart CA, Mcnamara JO 2nd, Thiel W, Giangrande P, Trinchieri G, Gilboa E. Isolation and optimization of murine IL-10 receptor blocking oligonucleotide aptamers using high-throughput sequencing. Mol Ther. 2012 Jun;20(6):1242-50. doi: 10.1038/mt.2012.18. Epub 2012 Mar 20. PMID: 22434135; PMCID: PMC3369303.

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