Aptamer Details

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RA16 (ID# 8056)

Aptamer Chemistry: RNA

Target: NCl-H460

Antigen/Target Category: Cells

Affinity (Kd): 24.75 ± 2.28 nM (reported value)

Binding Conditions/Buffer: 10× transcription buffer (400 mM Tris-Cl, 80 mM MgCl2, and 20 mM spermidine), 10 mM dithiothreitol, 20 U T7 mutant (Y639F) RNA polymerase, 10 mM ATP, 10 mM GTP, 10 mM 2′-F-dCTP/UTP, 2 mM 16-Biotin-UTP, 20 U RiboLock RNase Inhibitor, and 0.05 U inorganic pyrophosphatase. The resulting reaction mixture was treated with 2 μL DNase I at 37 °C for 1 h, followed by phenol-chloroform extraction.

Binding Temp: 37 °C°C

Refolding Protocol: RNA pellets were suspended in RNA refolding buffer (10 mM HEPES pH 7.4, 50 mM NaCl, 1 mM CaCl2,1 mM MgCl2, and 2.7 mM KCl), followed by refolding at 90 °C for 3 min and slowly cooling to room temperature.

Sequence ():

Length: 83

Molecular Weight: 26830.1

Extinction Coefficient: 826700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Wang, Hanlu, et al. “In Vivo SELEX of an Inhibitory NSCLC-Specific RNA Aptamer from PEGylated RNA Library.” Molecular Therapy - Nucleic Acids, Cell Press, 9 Dec. 2017, www.sciencedirect.com/science/article/pii/S2162253117303013.

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