Aptamer Details

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Ras DNA Aptamer Ra1 (ID# 8405)

Aptamer Chemistry: DNA

Target: Human Ras Protein

Antigen/Target Category: Protein

Affinity (Kd): 15.3 nM (reported value)

Binding Conditions/Buffer: 20 mM HEPES, pH 7.35, 120 mM NaCl, 5 mM KCl, 1 mM MgCl2, 1 mM CaCl2

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Sterile dsH2O (to remove non-specifically bound DNA) was used as a negative target control in SELEX section.

Comments: The coding sequences of Ra1 has been inserted into various vectors to generate aptamer in vivo, target to intracellular Ras protein and function as expected in mammalian cell lines and tumor cell lines. Note: All the properties of aptamer Ra1 including binding affinity, binding conditions, etc. has been obtained from a previous article which can be found in the reference section.

Sequence ():

Length: 78 bp

Molecular Weight: 51.48 kDa

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

[1] Ling N, Liu H, Guo J, Liang Z, Zhang Y, Li H, Wu H, Xie T, Yuan Y, Li X, Peng M, Wei X, Liang L, Liu J, Wu W, Ye M. Generation of DNA Aptamers with Functional Activity in Mammalian Cells by Mimicking Retroviruses. Anal Chem. 2023 Jul 4;95(26):9797-9804. doi: 10.1021/acs.analchem.3c00387. Epub 2023 Jun 16. PMID: 37327388. [2] Wang ZW, Wu HB, Mao ZF, Hu XP, Zhang H, Hu ZP, Ren ZL. In vitro selection and identification of ssDNA aptamers recognizing the Ras protein. Mol Med Rep. 2014 Sep;10(3):1481-8. doi: 10.3892/mmr.2014.2337. Epub 2014 Jun 16. PMID: 24938205.

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