Aptamer Details

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RhD antigen‑specificity 1 (ID# 8105)

Aptamer Chemistry: DNA

Target: RhD+ red blood cells

Antigen/Target Category: Cells

Affinity (Kd): 580.5±142.0 nM (reported value)

Binding Conditions/Buffer: 10 mM Tris-HCl, 50 mM KCl, pH 9.0), 1.5 mM MgCl2, 400 µM each dNTP, 2.5 U Taq DNA polymerase (Promega Corporation), and 50 pmol each biotinylated primer

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 40

Molecular Weight: 12280.9

Extinction Coefficient: 363800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Zhang Y, Xu H, Wang X, Wang L, Liu R, Li L, Zhou H. Single‑strained DNA aptamers mask RhD antigenic epitopes on human RhD+ red blood cells to escape alloanti‑RhD immunological recognition. Mol Med Rep. 2020 Apr;21(4):1841-1848. doi: 10.3892/mmr.2020.10985. Epub 2020 Feb 12. PMID: 32319623; PMCID: PMC7057830.

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