Aptamer Details

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Ricin A-chain (RTA) Aptamer (RA80.1.d2 – 31RA) (ID# 7953)

Aptamer Chemistry: RNA

Target: Ricin A-chain (RTA)

Antigen/Target Category: Protein

Affinity (Kd): 7.4 nM (reported value)

Binding Conditions/Buffer: 1X PBS, 5 mM MgCl2

Binding Temp: 23°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 31

Molecular Weight: 8089.29

Extinction Coefficient: 261800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

(1) Bogomolova, A., Aldissi, M. ƒ??Real-time and label-free analyte detection in a flow-through mode using immobilized fluorescent aptamer/quantum dots molecular switches.ƒ? Biosensors and Bioelecgtronics, 66 (2015): 290-296. (2) Hesselberth, J., et al. ƒ??In vitro Selection of RNA Molecules That Inhibit the Activity of Ricin A-chain.ƒ? Journal of Biological Chemistry, 275, 7 (2000): 4937-4942.

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