Aptagen, LLC
RNA aptamer-1-based sensitive detection of SARS-CoV nucleocapsid protein (ID# 8030)
RNA
SARS-CoV Nucleocapsid (N) Protein
Protein
1.65 nM (reported value)
30mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 2mM DTT, 1%BSA
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprGprAprGprAprGprCprGprGprAprAprGprCprGprUprGprCprUprGprGprGprCprCprUprGprUprCprGprUprUprCprGprCprUprGprUprGprUprCprUprUprGprCprUprAprCprGprUprUprAprCprGprUprUprAprCprAprCprGprGprUprUprGprGprCprAprUprAprAprCprCprCprAprGprAprGprGprUprCprGprAprUprGprGp-3'
85
20566.55
1021300
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Ahn, Dae-Gyun,et al. "RNA aptamer-based sensitive detection of SARS coronavirus nucleocapsid protein" The Royal Society of Chemistry, (2009):1896-1901.
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