Aptamer Details

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S. Enterica Serovar Typhi IVP Pili Protein (S-PS8.4) (ID# 7532)

Aptamer Chemistry: RNA

Target: S. Enterica Serovar Typhi IVP Pili Protein

Antigen/Target Category: Protein

Affinity (Kd): 7.2 nM (reported value)

Binding Conditions/Buffer: Binding Buffer (25 mM Tris-HCl, 50 mM KCl, 200 mM NaCl, 0.2 mM EDTA, 5% [vol/vol] glycerol, 0.5 mM DTT)

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 71

Molecular Weight: 22923.86

Extinction Coefficient: 696500

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Pan, Q., et al. "Aptamers That Preferentially Bind Type IVB Pili and Inhibit Human Monocytic-Cell Invasion by Salmonella enterica Serovar Typhi." Antimicrobial Agents and Chemotherapy, 49(2005): 4052-4060.

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