Aptamer Details

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S3-2-3 (ID# 8178)

Aptamer Chemistry: DNA

Target: Esophageal cancer cells.

Antigen/Target Category: Cells

Affinity (Kd): 265 nM (reported value)

Binding Conditions/Buffer: 4.5 g L−1 glucose, 5 mM MgCl2, 1 mg mL−1 bovine serum albumin (BSA) and 0.1 mg mL−1 yeast tRNA in 0.01 M PBS

Binding Temp: 4°C

Refolding Protocol: Denatured in binding buffer at 95 °C for 5 min and quickly cooled on ice for 10 min

Specificity: Binding to cell lines: KYSE150, other ESCCNo binding to: hepatocellular cancer cell line (SMMC-7721), gastric cancer cell line (SNU-5), lung cancer cell line (EBC-1), breast cancer cell line (MCF-7) and embryonic esophageal cell line (SHEE)

Sequence ():

Length: 18

Molecular Weight: 5710.7

Extinction Coefficient: 184000

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Chen, X., Zhang, Y., Shi, Y., Niu, T., Li, B., & Guo, L. et al. (2021). Evolution of DNA aptamers against esophageal squamous cell carcinoma using cell-SELEX. The Analyst, 146(13), 4180-4187. doi: 10.1039/d1an00634g

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