Aptamer Details

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S6-1 (ID# 8061)

Aptamer Chemistry: DNA

Target: human malignant glioma cell line SHG44

Antigen/Target Category: Cells

Affinity (Kd): 44 ± 15 nM (reported value)

Binding Conditions/Buffer: 4.5 g/L of glucose, 0.01 M phosphate buffered saline, and 5 mM of MgCl2

Binding Temp: 37 °C°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 60

Molecular Weight: 18474

Extinction Coefficient: 583800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Miyakawa, Shin, et al. "Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G." RNA Journal 14,(2008): 1154-1163.

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