Aptamer Details

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SARS-CoV-2 Aptamer 1C – Apta-switch antisense (ID# 8041)

Aptamer Chemistry: DNA

Target: RBD of the Spike glycoprotein from SARS-CoV-2

Antigen/Target Category: Protein

Affinity (Kd): 5.8 nM (reported value)

Binding Conditions/Buffer: 10 mM Tris, 0.5 mM NaCl, 1 mM EDTA, pH 7.4

Binding Temp: 23°C

Refolding Protocol: Main aptamer: 80°C for 3 minutes, gentle cooling at 23°C for 5 minutes. Antisense Apta-switch component: 40°C for 10 minutes, then combine with refolded main aptamer and incubate for 2 hours at 23°C. Wash with 1X DPBS, store at 4°C.

Comments: Apta-switch component (antisense strand) to SARS-CoV Spike protein aptamer (ID #: 7058). Originally reported with poly(dT) spacer and linker for functionalization.

Sequence ():

Length: 15

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Singh NK, Ray P, Carlin AF, Magallanes C, Morgan SC, Laurent LC, Aronoff-Spencer ES, Hall DA. "Hitting the diagnostic sweet spot: Pont-of-care SARS-CoV-2 salivary antigen testing with an off-the-shelf gluocometer". medRxiv preprint. doi: https://doi.org/10.1101/2020.09.24.20200394.

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