Aptamer Details

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Sp1 (ID# 8168)

Aptamer Chemistry: DNA

Target: Shigella sonnei

Antigen/Target Category: Cells

Affinity (Kd): 5.980 nM (reported value)

Binding Conditions/Buffer: 50 mM Tris–HCl, 5 mM KCl, 100 mM NaCl, 1 mM MgCl2, pH 7.4

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Highly Specific to the pathogenic enterobacteria Shigella sonnei

Sequence ():

Length: 40

Molecular Weight: 12061.73

Extinction Coefficient: 345300

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Gong, Wenhui, et al. “Selection, Identification, and Application of Dual DNA Aptamers against Shigella Sonnei.” Analytical Methods, vol. 7, no. 8, 2015, pp. 3625–3631., https://doi.org/10.1039/c5ay00214a.

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