Aptamer Details

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SP6 RNA polymerase (S05) (ID# 7673)

Aptamer Chemistry: RNA

Target: SP6 RNA Polymerase

Antigen/Target Category: Protein

Affinity (Kd): 9.5 nM (reported value)

Binding Conditions/Buffer: Binding Buffer (50mM Tris-HCl, pH 7.6, 1 M NaCl, 10mM 2-mercaptoethanol, 10% glycerol) at 4°C overnight at -20°C until use

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 35

Molecular Weight: 11178.67

Extinction Coefficient: 352800

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Mori, Y. (2012). Inhibitory RNA aptamer against sp6 RNA polymerase. Biochemical and Biophysical Resarch Communications, 420 (2012), 440-443.

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