Aptagen, LLC
Spinach (ID# 8022)
RNA
3,5-dimethoxy-4-hydroxybenzylidene imidazolinone (DHMBI)
Small Organic
512 nM (reported value)
40 mM HEPES pH 7.4 (or 40 mM Bicine pH 8.0), 125 mM KCl, 5 mM MgCl2, and 5% DMSO
23°C
Heat denatured at 75C for 5 min and quickly placed on ice to cool.
5'-rGprGprGprCprUprAprUprUprGprCprUprGprGprAprGprGprGprGprCprGprCprCprAprCprAprUprGprAprAprAprGprUprGprGprUprGprGprUprUprGprGprGprUprGprCprGprGprUprCprGprGprCprGprAprUprAprGprCprUprCp-3'
60
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Paige, J. S., Wu, K. Y., & Jaffrey, S. R. (2011). RNA Mimics of Green Fluorescent Protein. Science, 333(6042), 642–646. doi:10.1126/science.1207339
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