Aptamer Details

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Sulforhodamine B (Clone 73 – min) (ID# 7549)

Aptamer Chemistry: DNA

Target: Sulforhodamine B

Antigen/Target Category: Small Organic

Affinity (Kd): 190 nM (reported value)

Binding Conditions/Buffer: Selection Buffer: 0.1 M KCl, 5 mM MgCl₂, 10 mM Na-HEPES (pH 7.4).

Binding Temp: RT°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Kd value listed is for sulforhodamine B immobilized to a column. Free sulforhodamine B binds with Kd= 660 nM.

Sequence ():

Length: 29

Molecular Weight: 9155.94

Extinction Coefficient: 284400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Wilson C, Szostak J. (1998) Isolation of a fluorophore-specific DNA aptamer with redox activity. Chem Biol. 5(11):609-617.

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