Aptamer Details

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SW1A (ID# 8183)

Aptamer Chemistry: DNA

Target: SMMC-7721 cells (liver cancer)

Antigen/Target Category: Protein

Affinity (Kd): 123.62 ± 17.53 nM (reported value)

Binding Conditions/Buffer: Washing Buffer: 0.01 M DPBS, 5 mmol/L MgCl2, and 4.5 mg/mL glucose Blocking Buffer: Washing Buffer with additional 0.1 mg/mL yeast tRNA, 1 mg/mL BSA, and 0.5 mg/mL salmon DNA (Solarbio) Binding Buffer: Washing Buffer with additional 1 mg/L bovine serum albumin (BSA, Dingguo) and 0.1 mg/mL yeast tRNA (Sigma)

Binding Temp: 37°C

Refolding Protocol: The initial ssDNA library (5 nmol) was dissolved in 100 μL of binding buffer and then heated at 95 °C for 5 min and immediately cooled on ice for 10 min

Sequence ():

Length: 35

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Li, L., Wan, J., Wen, X., Guo, Q., Jiang, H., Wang, J., … Wang, K. (2021). Identification of a New DNA Aptamer by Tissue-SELEX for Cancer Recognition and Imaging. Analytical Chemistry, 93(19), 7369–7377. doi:10.1021/acs.analchem.1c01445

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