Aptamer Details

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T20 (ID# 8233)

Aptamer Chemistry: DNA

Target: Tetracycline

Antigen/Target Category: Small Organic

Affinity (Kd): 63.6 nM (reported value)

Binding Conditions/Buffer: Briefly, the target chemical-coated magnetic beads were washed several times with binding buffer containing NaCl (100 mM), Tris–HCl (20 mM, pH 7.6), MgCl2(2 mM), KCl (5 mM), CaCl2(1 mM), and Tween 20 (0.02%) before each round of SELEX process.

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Specific to tetracycline (TET) nucleus of tetracycline antibiotics. The aptamer binds to multiple different tetracyclines such as oxytetracycline (OTC) and doxycycline (DOX). Aptamers specific to one target molecule but not the other structurally related molecules were thrown out in order to promote specificity to the desired central structure.

Comments: Reference paper shows sequences and structures for related aptamers and discusses motifs.

Sequence ():

Length: 76

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Niazi, J. H., Lee, S. J., & Gu, M. B. (2008). Single-stranded DNA aptamers specific for antibiotics tetracyclines. Bioorganic & medicinal chemistry, 16(15), 7245–7253. https://doi.org/10.1016/j.bmc.2008.06.033 https://pubmed.ncbi.nlm.nih.gov/18617415/

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