Aptamer Details

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TAT RNA Aptamer TAT-X (ID# 8412)

Aptamer Chemistry: RNA

Target: Recombinant Trans-Activator of Transcription Aptamer binding Protein (RBP-mCherry-RBP)

Antigen/Target Category: Protein

Affinity (Kd): 3.49 nM (reported value)

Binding Conditions/Buffer: 50 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.4.

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Four TAT aptamers were fused together through a specially designed four-way junction and the corresponding RNA transcript was referred to as TAT-X which was produced stably in vivo and readily isolated from RNase III-deficient cells. With specific modification, TAT aptamer can also be conjugated with other aptamers including Spinach 2 to produce more multimeric aptamers with related functions.

Sequence ():

Length: 204 nt

Molecular Weight: 67.32 kDa

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Zou Z, Younas T, Dumsday G, Haritos VS, He L. Rapid production of multimeric RNA aptamers stabilized by a designed pseudo-circular structure in E. coli. Biotechnol J. 2023 Mar;18(3):e2200390. doi: 10.1002/biot.202200390. Epub 2022 Dec 5. PMID: 36427490.

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