Aptamer Details

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Theophylline (mTCT8-4) (ID# 7551)

Aptamer Chemistry: RNA

Target: Theophylline

Antigen/Target Category: Small Organic

Affinity (Kd): 100 nM (reported value)

Binding Conditions/Buffer: Buffer A (100 mM Hepes (pH 7.3), 5 mM MgCI2, and 0.5 M NaCI)

Binding Temp: N/A°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Truncated version from Figure 2 in paper. Arrows indicate truncation. Binds to theophylline with 10,000 times greater affinity than caffeine, which differs only by a methyl group.

Sequence ():

Length: 38

Molecular Weight: 12097.29

Extinction Coefficient: 364600

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Jenison et al. "High-resolution molecular discrimination by RNA." Science, 263(1994): 1425-1429.

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