Aptamer Details

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Thrombin (15mer-29-mer dimer with 20T linker) (ID# 7812)

Aptamer Chemistry: DNA

Target: Thrombin

Antigen/Target Category: Protein

Affinity (Kd): 0.35 nM (reported value)

Binding Conditions/Buffer: Binding Buffer: 50 mM Tris-HCl, 300 mM NaCl, 5 mM KCl, pH 8.0.

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: This aptamer is a dimerized version of the 15-mer and 29-mer thrombin aptamers connected by a 20-mer thymidine linker. This aptamer showed improved affinity and inhibitory effects to the monomer components.

Sequence ():

Length: 64

Molecular Weight: 19958.03

Extinction Coefficient: 588600

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

H. Hasegawa et al. Improvement of aptamer affinity by dimerization. Sensors 8(2008): 1090-1098.

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