Aptamer Details

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Thrombin (31mer) (D2-5) (ID# 7787)

Aptamer Chemistry: DNA

Target: Thrombin

Antigen/Target Category: Protein

Affinity (Kd): N/A nM (reported value)

Binding Conditions/Buffer: Buffer for Clotting Inhibition assay: Tris-HCl pH 8.0, 5 mM KCl, 100 mM NaCl.

Binding Temp: 37°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: Aptamer consists of a stem-loop (see figure) with the loop forming a G-quartet identical to the 15-mer thrombin-binding aptamer in the Related Aptamers section below. The authors optimized the stem for clotting inhibition.

Sequence ():

Length: 31

Molecular Weight: 9710.34

Extinction Coefficient: 295700

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

K Ikebukuro et al. Analysis of the evolution of the thrombin-inhibiting DNA aptamers using a genetic algorithm. Biotechnol Lett (2006) 28:1933ƒ??1937.

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