Aptamer Details

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Thrombin Aptamer 2 (ID# 8160)

Aptamer Chemistry: DNA

Target: Thrombin

Antigen/Target Category: Protein

Affinity (Kd): 0.027 nM (reported value)

Binding Conditions/Buffer: TE (20 mM Tris-HCl, 140 mM NaCl, 5 mM KCl, 5 mM MgCl2 and 1mM CaCl2, pH 7.4)

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Comments: 5'SH-(CH2)6-TTTTTTTTTTTTTTTTTTTTAGTCCGTGGTAGGGCAGGTTGGGGTGACT '3

Sequence ():

Length: 147

Molecular Weight: 15448.88

Extinction Coefficient: 435546

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

doi:10.1039/C7TB01276D

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